Artificial intelligence (AI) can improve efficiency, reduce administrative load, and improve clinical care, but the successful uptake of AI requires nurse engagement and training. This paper examines nurses’ perceptions of AI across different clinical settings. This secondary data analysis uses the 2023 Canadian Survey of Nurses, with n=1196 clinical care nurses. Only 38 (3.2%) nurses reported that AI is being used in their care setting. There was a negative correlation between level of comfort with AI being used in healthcare (rs=-.073, p=.017) and how knowledgeable respondents felt about AI (rs=-.171, p<.001) based on the number of years of experience in nursing. More recent graduates may be more knowledgeable about AI, however, most report a knowledge gap and discomfort with AI. Future studies should focus on exploring more deeply what training and knowledge needs nurses have around the use of AI in their practice.